The Accellix Automated Flow Cytometer: Reproducible and Efficient Phenotyping for Cell Therapy Manufacturing
Light scatter in the forward (FSC) and side (SSC) directions in flow cytometry provides valuable insight into relative particle size and internal complexity. The Accellix Platform, a harmonized and automated cytometer engineered for cell and gene therapy manufacturing and quality control, does not collect SSC, and the scatter gate is replaced with a FSC by Peak Time biplot during analysis. We report the relationship between FSC and particle size on the Accellix instrument to guide users in setting the FSC limit during post-acquisition data analysis.
To demonstrate reproducibility and efficiency, Accellix, in collaboration with Triumvira, conducted a study that aims to demonstrate the ease of use, high reproducibility and comparability of the Accellix platform to conventional cytometers and cell counters.
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