Concurrent Enumeration of CD34+ and CD3+ Cells in Allogeneic Apheresis Collections Using a New Flow Cytometry Method

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Authors: Elaine Than², Yesmin Chowdhury¹, Alla Oren¹, Rehana Clouden¹, Marina Zlotina¹, Felix Montero-Julian², Hemant Raj Joshi¹, Wei Cai¹, Ahmad Al-Attar³, Gal Gabay² and Yossef Vasilevski², Amanda Trent² and Camelia Iancu-Rubin¹
¹Icahn School of Medicine and Mount Sinai Hospital, Mount Sinai Health System, New York, NY, USA; ²Accellix, Inc. San Jose, CA, USA; ³University of Pittsburgh, Pittsburgh, PA, USA

ISCT 2026 | May 6 – 9 | Dublin, Ireland

Description: Donor lymphocyte infusions (DLI) are commonly used to prevent or treat relapse after allogeneic stem cell transplantation. DLIs may be collected through a separate apheresis procedure or derived from excess mobilized stem cell grafts. The latter approach is routine at our institution and requires quantification of both CD34⁺ stem/progenitor cells and CD3+ T lymphocytes. Currently, these measurements are performed in two separate assays by an independent laboratory. Here, we evaluated a new flow cytometry method that enables in-house simultaneous enumeration of CD34⁺ and CD3⁺ cells in a single assay with the potential to streamline graft characterization, reduce turnaround time, and decrease resource utilization.

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Cell Therapy Manufacturing QCStem Cell Transplant Enumeration & PhenotypingBlood Donor Phenotyping

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Concurrent Enumeration of CD34+ and CD3+ Cells in Allogeneic Apheresis Collections Using a New Flow Cytometry Method

Provide the following information and we’ll email you a copy of the poster:

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