
Evaluating the Automated Accellix® Platform Against Conventional Flow Cytometry for Cell Therapy Samples
Description: Cell therapy manufacturing requires rapid, reliable cell phenotyping to guide critical process decisions. However, conventional flow cytometry often presents challenges including lengthy turnaround times, complex workflows and the need for specialized expertise in Flow cytometry. This poster presents a comparison between the Accellix Platform using off-the-shelf assays and conventional flow cytometry across representative cell therapy samples. We evaluated:
- 3 lots of cryopreserved PBMCs (representing incoming starting material)
- 3 lots of PBMCs treated with T-cell activation reagents (representing in-process samples)
- 3 lots of cryopreserved CD19 CAR-T drug product (representing pooled/final drug product)
- Streck CD-Chex Plus® control material to verify assay performance and allow for standardization and validation
Our results demonstrate that the Accellix platform achieves data quality comparable to conventional methods while substantially reducing turnaround time and operational complexity.
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