Each kit comes with a single-use microfluidic cartridge and an assay-specific dried reagent tube containing all necessary components to stain a cell sample. The operator simply adds the cell sample to the tube, mixes for 2 minutes on a pulse vortexer (included with instrument) to reconstitute the dried reagents, then pipettes the sample into the cartridge. In addition, each reagent tube also contains fluorescent control beads that serve two functions:
ratio-metric counting of cells, providing total cells/mL, and
in-run instrument performance check.
Are customized assays available?
Yes, we specialize in migrating custom flow cytometry assays to the Accellix platform. Any assay panel with up to 6 different fluorophores can be developed into a custom assay kit, allowing you to bring the power of flow cytometry to the manufacturing floor.
What is the stability of each kit?
12 months at room temperature (18C – 22C). No cold chain required.
How much sample does it take to run an assay?
The operator adds 40uL of sample to the dried reagent tube, mixes for 2 minutes to reconstitute the reagents, then pipettes 10-25µl into the cartridge.
What range of cell concentrations can be run on the Accellix?
We recommend the customer use samples at 10-20 million cells/mL. The instrument can handle samples from 1-50 million cells/mL. The cell concentration used depends on the frequency of the population of interest. Low frequencies will require a higher event count, which can be achieved by using more concentrated cell samples or by configuring the cartridge protocol to use a lower sample dilution.
How many cell events are typically detected in a run?
This is sample- and assay-dependent, but a typical range of total cell events is 20k-100k. For dilute samples or low-frequency cell subsets, cell event count can be increased by increasing the sample volume added to the cartridge (max of 25 uL), decreasing the dilution within the cartridge, and/or increasing acquisition time.
What sample types can be used?
Many sample types can be used on the Accellix platform, including whole blood, apheresis samples and purified cells. The cells can be in a wide range of matrices such as cell culture media, commercial staining buffers, PBS, and in some cases cryo-preservation media.
What input is needed from the operator to run an assay?
The Accellix platform automates the entire process, from sample preparation to spectral compensation and voltage settings, to auto-analysis. After reconstituting the dried reagents with the cell sample by mixing on a pulse vortexer for 2 minutes, the operator pipettes the sample into the cartridge. Upon inserting the cartridge into the instrument, the operator is prompted to enter a sample ID. From this point on, the instrument takes over.
How does the instrument know which assay protocol to run?
Every cartridge has a unique QR code that is read automatically by the instrument. It contains all relevant information for that specific run, including assay type, serial number, lot number and expiration date. Using the QR code, the instrument will execute the appropriate assay protocol.
How many assays is each cartridge able to perform?
Each individual assay kit contains everything needed for automated sample preparation, data acquisition and analysis of one cell sample, returning valuable time back to the operator.
What is the turnaround time per run?
30 minutes for most assays.
What happens to the sample once it’s loaded into the cartridge?
Actuators within the instrument compress and release two individual bellows on the cartridge, enabling controlled movement of the sample through the microfluidic channels and mixing chambers. Three blisters on the cartridge containing assay-specific buffer are used to dilute and fix the stained cells. Upon completion of all incubations and dilutions, the sample is moved through a 50 µm diameter channel within the flow cell region of the cartridge, where cell fluorescence and forward scatter signals are then detected.
Do we need antibody titration for the N-1 and N-2 cartridges?
The n-1 and n-2 kits are intended for use as proof of concept or testing feasibility of an assay by adding one or two conjugates by the customer. We recommend that the customer test the antibody conjugate at the titer recommended by the vendor. You may titrate the antibody at your end if you are not obtaining desired results. Once you demonstrate basic feasibility, customers typically move towards a custom development project. We can provide you with all reagents in dried down format for your use at the end of phase 1 development. This will allow you to have a customized kit with minimal operator input and reproducible results from run to run.